Endothelin Assay in Development for the Sgx Clarity^TM System Detects ET in Plasma and Discriminates Chronic Heart Failure from Healthy Donor Samples

A. Bartolome, S. Biscocho, J. Stiles, U. Enkhbayar, R. Ramirez, R. Sweetwood, T. Dang, H. Morrell, K. Bui, A. Mukherjee, J. Estis, R. Livingston, L. Monsalve, J. Bishop, J. Felberg, J. Todd, Singulex, Inc. Alameda, CA

BNP Assay in Development for the Sgx Clarity^TM System Demonstrates Increased Clinical Sensitivity Relative to a Conventional BNP Immunoassay

X. Wang, S. Tjon-Kon-Sang, V. Torres, R. Livingston, L. Monsalve, J. Todd, J. Felberg, J. Bishop, Singulex, Inc. Alameda, CA

Characterization of Plasma Endothelin-1 in a Large Community-based Patient Population at Risk for Cardiovascular Disease

A. H. Wu, J. Estis, P. Heseltine, T. Dang, J. Todd, P. Kavsak, University of California, San Francisco, San Francisco, CA, Singulex, Inc., Alameda, CA, McMaster University, Hamilton, ON, Canada

High-Sensitivity Cardiac Troponin I in a Large Community-Based Population at Risk for Cardiovascular Disease

A. H. Wu, J. Estis, P. Heseltine, K. Bui, J. Todd, P. Kavsak, University of California, San Francisco, San Francisco, CA, Singulex, Inc., Alameda, CA, McMaster University, Hamilton, ON, Canada

The Relationship of Inflammatory Cytokines (IL-6, IL-17A, and TNF-α) to Co-morbidities of Cardiovascular Disease in a Large Community-based Patient Population

A. H. Wu, J. Estis, P. Heseltine, J. Todd, P. Kavsak, University of California, San Francisco, San Francisco, CA, Singulex, Inc., Alameda, CA, McMaster University, Hamilton, ON, Canada

Reference Range Study Using a High Sensitivity cTnI Assay in Development for the Sgx Clarity^TM System

L. Shephard, S. Florey, Y. Cheung, R. Livingston, Luis Monsalve, J. Todd, J. Bishop, J. Felberg, Singulex, Inc. Alameda, CA

Relationships of QT interval with cardiac biomarkers in young adults

Kofler T, Bossard M, Aeschbacher A, Maseli A, Blum S, Todd J, Estis J, Risch M, Risch L, Conen D

Determinants of plasma kidney injury molecule-1 and its prognostic utility in patients with acute heart failure

Sokolski M, Zymlinski R, Biegus J, Siwolowski P, Jankowska EA, Todd J, Yerramilli R, Estis J, Banasiak W, Ponikowski P

Increase in ultra-sensitive troponin I is associated with poor prognosis in patients with acute heart failure

Zymlinski R, Sokolski M, Biegus J, Siwolowski P, Jankowska EA, Todd J, Yerramilli R, Estis J, Banasiak W, Ponikowski P

Plasma KIM1 in Heart Failure renal mechanisms and clinical outcome

Emmens J, M.ter Maaten J, Matsue Y, Metra M, O’Connor C, Ponikowski P, Teerlink J, Cotter G, Davison B, Cleland J, Givertz M, Bloomfield D, Dittrick H, Todd J, van Veldhuisen D, Hillege H, Damman K, van der Meer, P, Voors AA

Biomarker Quantification Improvements for ELISA Conversion to an Erenna® Immunoassay

Robert Goodson, Lynn Zieske, Sarah Hamren, Joseph Barco, Singulex, Inc., Alameda, CA

Use of the Erenna Immunoassay Platform to detect pro-inflammatory cytokines in an in vitro cytokine release assay

S. Teixeira*, S. Salgado-Pires*, Z. Johnson*, M. Deehan*, A. Lu§, L. Zieske§, W. Ferlin*, F. Guilhot* *NovImmune SA, Geneva, Switzerland; § Singulex, CA, USA

A review of high sensitivity digital immunoassays in PK/PD studies

Lynn Zieske, Singulex, Inc., Alameda, CA

Cytokine and Inflammation 2012

J. Greenberg1, V. Furer1, Q.A. Lu2, R. Ramirez2, S. Abramson1, M. Att ur1, J. Hsu2, L. Zieske2 1NYU Hospital for Joint Diseases, New York, NY. 2Singulex, Inc., Alameda, CA
10th Cytokines and Infl ammati on Conference 2012 San Diego, CA

2011 The 9th Cytokines & Inflammation Conference

Jenny Hsu, Sara J. Agee and Lynn Zieske. Singulex, Inc., Alameda, CA

AACR 2011 -Elevations of Intracellular Kinases AKT1, GSK3_, JNK2 and ERK2 in Primary Breast Cancer Tissue Quanti_ed with a Novel Multipanel of Single Molecule Immunoassays

Sara Agee, Joel Esti s, Jenny Hsu, Quynh Ann Lu, John Todd and Lynn Zieske. Singulex, Inc., Alameda, CA

AAPS NBC 2011 -- Method Development and Validation of an Alternative Immunoassay Platform for Pharmacokinetic Studies in a Regulated Environment

Lisa Dyleski, Alison Joyce, Denise M. OêHara, Shobha Purushothama, Kathleen Chapman, Bruce Holmes Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Andover, MA

EULAR 2011 -- Changes in Plasma Biomarker Panel of Cardiovascular Risk with Biologic Agents in Rheumatoid Arthritis

J. Greenberg 1,*, V. Furer 1, J. Todd 2, S. Abramson 1, Q. A. Lu 2, R. Ramirez 2, M. Attur 11NYU HOSPITAL FOR JOINT DISEASES, New York, 2Singulex, Alameda CA, United States

2011 The 3rd Annual Assay Development Conference -- Single-molecule quantification of protein biomarkers with the Erenna® Immunoassay System

Jenny Hsu, Sara J. Agee and Lynn Zieske. Singulex, Inc., Alameda, CA

EB2011 -- Baseline Serum Cardiac Troponin I Concentrations in Various Strains of Rats asDetermined with an Ultrasensitive Immunoassay

Eugene Herman_, Alan Knapton_, Elliot Rosen_, Quynh-Ahn Lu_, Joel Estis_, Sara Agee_, John Todd_, Steven Lipshultz_, Jun Zhang__FDA, Silver Spring, MD 20993, _Singulex Inc, Alameda, CA 94502, _University of Miami, Miami, FL, 33101

EB2011 -- An Assessment of Skeletal Muscle Troponin I as a Biomarker of Drug-Induced Skeletal Muscle (SM) Toxicity

Alan Knapton1, David Peters1, Elliot Rosen1, Quynh-Anh Lu2, Joel Estis2, Sara J. Agee2, John H. Todd2, Eugene H. Herman11DAPR, CDER, FDA, Siver Spring, MD 20993. 2Singulex Inc, Alameda, CA, 94502

AACR-TCM 2010 - Elevations of Intracellular Kinases AKT1, GSK3Β, JnK1, JnK2 and ERK2 in Primary Breast Cancer Tissue Quantified with a Novel Multipanel of Single Molecule Immunoassays

Joel Estis, Jenny Hsu, Quynh Anh Lu, Lynn Zieske, John Todd and Sara J. Agee. Singulex, Inc., Alameda, CA

2010 First International Conference on Cancer and the Heart -- Imatinib Cardiotoxicity in Hypertensive and Normotensive Rats

Eugene Herman1, Alan Knapton1, Elliot Rosen1, Quynh Anh Lu2, Joel Estis2, John Todd2, Steven Lipshultz3, Jun Zhang11DAPR,CDER, FDA, Silver Spring MD 20993, 2Singulex Inc, Alameda, CA 94502, 3University of Miami School of Medicine, Miami FL 33136.

SOT 2010 -- Serum cardiac troponin I concentrations are transiently increased in rats dosed with rosiglitazone, a peroxisome proliferator-activated receptor γ agonist

G. Hirkaler1, R. Fernandes1, D. Coluccio1, C. Kanwal1, W. Geng1, E. Rasmussen1, T. Visalli1, M. O. Bachynsky1, H. Hilton1, R. Nicklaus, J.-C. Hoflack2, A. Buness2,F. Herting3, L. Suter Dick4, I. Mikaelian1SOT: 1308-805 Hoffmann-La Roche Inc., 1Nutley, NJ, 07110; 2Basel, Switzerland, CH-4070; 3Penzberg, Germany, DE-82377.

AHA 2010 -- A Multi-center Comparison of Established and Emerging Cardiac Biomarkers for the Diagnostic Evaluation of Chest Pain in the Emergency Department

AHA 2010 -- Multiple Biomarkers for the Prediction of Cardiovascular Death Within the Minnesota Heart Survey

AHA 2010 -- Patients with Rheumatoid Arthritis Have Elevated High-Sensitivity Cardiac Troponin-I Concentrations Independent of Cardiovascular Risk Factors and Inflammation

AACC 2009 -- Serum 99th Percentile Reference Value for the High Sensitive Singulex Cardiac Troponin I Assay

Fred S. Apple1 « MaryAnn M. Murakami1 « Denise P. Farris1 « Saker A. Karimi2 « Peter A. Simpson2 « Loan T. Le21Hennepin County Medical Center, Minneapolis MN « 2Singulex, Alameda CA

AACC 2009 -- Performance Characteristics of the Erenna cTnl Assay for In Vitro Diagnostics Use in the Singulex Clinical Laboratory

Saker Karimi, Peter Simpson, Khanh Bui, Emerson Quan, Loan Le, Jenny Hsu, Sara Agee and Guido BaechlerSingulex, Inc. Alameda, CA, USA

FABS 2009 -- Bridging the Gap Between Pre-clinical and Clinical Assay Validation: the Erenna® Immunoassay System

Lynn Zieske, Joel Estis, Jennifer Minyard, Quynh A. Lu, Jenny Hsu, Sara J. Agee, John ToddSingulex, Inc. Alameda, CA, USA

AACC 2009 -- Harmony of cTnI Analysis Across Six Commercially Available Platforms

Oak Ridge Conference 2008 - Use of Single-molecule Counting Technology to Determine the Concentration of Cytokines IL-6, IL-8, IL-17, and IL-1B in Plasma from Healthy Subjects.

Lu A, Agee SJ, Held D, Rieckenberg C, Freese R, Sheets D, Livingston R, Puskas R, Todd J
Singulex Inc., Alameda, CA

ADA 2008 -- Development of a High Sensitivity Assay for Mouse Plasma Insulin Using Single Molecule Counting.

P.Gilmore1, K.Mills2, C.Brunkan2, B.Klein4, R.Townsend1, S-I. Imai2, V.Luzzi3, 1Washington University Proteomics Core Lab, 2Department of Developmental Biology, and 3Core Lab for Clinical Studies at Washington University School of Medicine, St. Louis, MO, 4Singulex Inc., Alameda, CA.

AACC 2008 -- Temporal Variability of Cardiac Troponin-I Concentrations in Rats Under Standard Laboratory Conditions

Albert E. Schultze1, Robert J. Konrad1, Quynh A. Lu2, Sara J. Agee2, John Todd21Lilly Research Laboratories, Greenfi eld, IN, 2Singulex, Inc., Alameda, CA

AACC 2008 -- Comparative Analysis Of An Ultra-sensitive Assay For Mouse Plasma Insulin Using Single Molecule Counting Technology

V. I. Luzzi1, P. Gilmore2, K. Mills2, C. Brunkan2, S. Imai2, R. Townsend2, B. K. Klein3, S. J. Agee3, J. Todd31Henry Ford Hospital, Department of Pathology, Detroit, MI; 2Washington University School of Medicine, St. Louis, MO; 3Singulex, Inc., Alameda, CA

AACC 2008 -- Detection of Transient Myocardial Ischemia Using a Highly Sensitive, Single Molecule, Troponin I Assay

Petr Jarolim,1 Marc S. Sabatine,1 David A. Morrow,1Stacy E.F. Melanson,1 Quynh Anh Lu,2James A de Lemos,3 John A Todd,2 Eugene Braunwald,11Brigham and Womenês Hospital, Harvard Medical School, Boston, MA,2 Singulex Inc., Hayward, CA, 3 University of Texas Southwestern Medical Center, Dallas, TX

AACC 2008 --Specificity of a New, Highly Sensitive Human cTnI Assay Utilizing Microparticles and Single Photon Fluorescence Detection

A. H. Wu1, A. Q. Lu2, S. J. Agee2, J. Todd2, A. S. Jaff e31University of California San Francisco, San Francisco, CA, 2Singulex, Inc., Alameda, CA, 3Mayo Clinic, Rochester, NY

AACC 2008 -- Short- and Long-term Biological Variation for Cardiac Troponin I Using a High Sensitivity Assay: Implications for Clinical Practice

Alan H.B. Wu,1Andrew Smith,1 Katherine Schultz,1Ann Lu,2John Todd,2Frank Wians31University of California, San Francisco, 2Singulex Inc., 3University of Texas Southwestern Medical Center, Dallas

ASCO 2008 -- Preliminary Validation of Two Novel Immunoassays for Detecting VEGF in Human and Mouse Plasma Using Single Molecule Counting Technology

Robert Puskas*1, Douglas Held1, Damon Sheets1, Barbara Klein1, Elizabeth Macy1, Sara Agee2, John Todd21Singulex, Inc., St. Louis, MO; 2Singulex, Inc., Alameda, CA

Bio2008 -- The Erenna Immunoassay System: Enabiling New Clinical Diagnostic Information through Single-Molecule Counting Technology.

AACC 2007 -- Development and Preliminary Clinical Validation of an Ultra-sensitive Assay for Cardiac Troponin Using Microparticle Based Immunoassay and Single Molecule Counting

Alan H.B. Wu,1 Ann Lu,2 Bob Freese,2 John Todd,1University of California, San Francisco, and2Singulex, Inc.

Oak Ridge Conference 2006 --Development and Preliminary Clinical Validation of a High Sensitivity Assay for Cardiac Troponin Using a Capillary Flow (Single Molecule) Fluorescence Detector

Alan H.B. Wu,1 Noelle Fukushima,2 Robert Puskas,2 John Todd,2 Philippe Goix21University of California, San Francisco, 2Singulex Inc., Berkeley, CA