Home  |  Contact Us  |  Subscribe  | About Us

Life Science Products
Assay Menu
Erenna® Technology
Instrumentation
Sgx link™
Custom Services
Product Literature
Contact Us
 

Immunoassays | Prototype

Erenna® Active GLP-1 (MP-based) Immunoassay Prototype Assay (Cat# 03-0024-AA)

download PDF data sheet

Introduction

The Erenna® Active GLP-1 Immunoassay Evaluation Reagent Kit uses a quantitative fluorescent sandwich immunoassay technique to measure Active GLP-1 plasma and serum samples. A capture antibody specific for the C-terminus of 7-36 (amide) and 9-36 (amide) GLP-1 has been pre-coated onto paramagnetic microparticles (MP). The user pipettes MP, standards, and samples into uncoated microplate wells. During incubation, any GLP- 1 present in the sample binds to the capture antibody on the coated MP. Unbound molecules are washed away during the subsequent buffer exchange and wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to the N-terminus of the Active GLP-1 that has been captured onto the MP. During the following wash step the MP’s are transferred to a clean plate. Elution buffer is then added and incubated. The elution buffer dissociates the bound protein sandwich from the MP surface, releasing the labeled antibodies. These antibodies are separated during transfer to a final Microplate. The plate is loaded into the Erenna System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of Active GLP-1 present in the sample when captured. The amount of Active GLP-1 in unknown samples is interpolated from a standard curve.

Assay Performance


TABLE 1. Analytical sensitivity of the Erenna® Active GLP-1 (MP-based) Immunoassay Prototype Assay1
Lower Limit of Detection 0.04 pg/mL
Lower Limit of Quantification2 0.2 pg/mL
Upper Limit of Quantification 50 pg/mL
Low-end CV% Range 2 - 12%
Low-end CV% Average 6%
Recommended Sample Volume 100 μL
Minimum Sample Volume Required3 10 μL

1 See product insert for updated values
2 LLoQ = 20% CV and ± 20% recovery
3 based upon median [GLP-1, active (MP-based)] in a healthy reference


FIGURE 1. [Active GLP-1] in EDTA plasma (collected in p700 tubes) from 5 healthy subjects, with respective median and interquartile range. The Erenna® Active GLP-1 Immunoassay Evaluation Reagent Kit reliably quantifies Active GLP-1 in EDTA plasma from healthy subjects, who have a median [Active GLP-1] of 2.15 pg/mL that is well above the detection limit of 0.04 pg/mL.

FIGURE 2. Erenna® Active GLP-1 Immunoassay Evaluation Reagent Kit low-end standard curve signal (left) and curve fit (right).

Representative data shown for demonstration purposes only. Individual results may vary depending upon samples tested and protocol used.

Biology and Disease

Glucagon-Like Peptide-1 (GLP-1) is a member of the incretin family of hormones, which are released by the gut into the bloodstream in response to food. Post-translational cleavage of the peptide produces active GLP-1, which is then rapidly degraded by protease dipeptidyl peptidase-4 (DPP-4). When blood glucose level is high, active GLP-1 enhances insulin secretion and also suppresses the release of glucagon. In addition, GLP-1 stimulates the brain to trigger feelings of satiety and regulates the rate of gastric emptying. Compared to healthy controls, some patients with type-2 diabetes show a modest decrease in GLP-1 release. Therapeutic GLP-1 analogs such as exenatide, or DPP inhibitors like sitagliptin, are promising strategies for treating type-2 diabetes.


TABLE 2. Additional UniProtKB/Swiss-Prot Information*
Protein Name:
UniProtKB/Swiss-Prot ID:
Protein-protein interaction database:
Alternative Names:
Gene Names:
Synonym Gene Names:
Function:
Biological Processes:
Molecular Function:

* Source Info from www.uniprot.org.


Related Products

Analyte Description  Catalog #
Aβ-40 Erenna® Aβ-40 Immunoassay Prototype Assay  03-0020-AA
Aβ-42 Erenna® Aβ-42 Immunoassay Prototype Assay  03-0021-AA
AKT1, phospho Erenna® pAKT1 Immunoassay Prototype Assay  03-0035-AA
AKT1, total Erenna® tAKT1 Immunoassay Prototype Assay  03‐0036‐AA
EGFR Erenna® EGFR Immunoassay Prototype Assay  03-0037-AA
IL-7 Erenna® IL-7 Immunoassay Prototype Assay  03-0054-AA
Insulin (human, MP-based) Erenna® Human (MP-based) Insulin Immunoassay Prototype Assay  03-0041-AA
MMP-2 Erenna® MMP-2 Immunoassay Prototype Assay  03-0044-AA
MMP-2/TIMP-2 complex Erenna® MMP-2/TIMP-2 Immunoassay Prototype Assay  03-0045-AA
TIMP-2 Erenna® TIMP-2 Immunoassay Prototype Assay  03-0046-AA
TNFα (mouse) Erenna® Mouse TNFα Immunoassay Prototype Assay  03-0060-AA

TERMS OF USE  |  PRIVACY POLICY